Scientific Research........................ Advancing Global Knowledge.



For Authors

Publication Ethics

Peer Review

Call For Paper

Processing Charges

Submit Manuscript





Related Journal

GSJ of Microbiology.



Global Scholars Journal of Biological Sciences Vol.2 (1), pp 7-16 November, 2016
©2016 Global Scholars Journals.


Full Length Research Paper

Effect of Nitrogen fertilizer on the in-vivo nitrate reductase activity in Dalbergia sissoo plants under nursery conditions

Poornima Uniyal, Satya Prasad Chaukiyal*, Vandana and Jyoti Kandpal

Plant physiology unit, Botany division, Forest research institute Dehradun, Uttarakhand, India.


Accepted 21 November, 2016.





Dalbergia sissoo is multipurpose Nitrogen (N) fixing tree species cultivated extensively in the Indian plains. It accumulates a considerable amount of N-rich litter which decomposes quickly and improves the organic matter as well as the nutrient status of the soil. It is generally recommended for afforestation of degraded lands in arid and semiarid regions, where soil N status is poor. N is one of the most important elements for the growth and development of plants.  In the present communication, an attempt was made to study the N assimilation behavior of individual plant parts in D. sissoo under nursery conditions. Urea was used as the N source and applied at 0, 50,100 and 200 Kg/ha in two split doses in pot grown plants. The objective was to observe the effect of N application on the in-vivo nitrate assimilation pattern in different plant parts of D.sissoo at monthly interval.
The results reveal that two peaks, i.e. one large in April and another small in August were observed in all four N treatments for leaf, stem, and root nitrate reductase (NR) activity (g-1 fresh wt h-1). However, in nodule two peaks one large in April and another small in July was observed. In total leaf (NR) activity (pl-1h-1), a large peak was observed in August and a small in June. In stem, large and small peak were observed in April and August respectively in all the treatments. Whereas in the root, a large peak in April and small in August were observed in both g-1 fresh wt h-1 and pl-1h-1 NR activity in all treatments. In leaf, stem and root higher NR activity was observed in 200 kg N/ha as compared to other treatments. However, in the nodule, maximum activity was observed in 50 kg N/ha as compared to others. On the basis of seasonal effect, higher NR activity (g-1 fresh wt h-1) was recorded in summer followed by rainy and minimum in the winter season in different plant parts. The total in-vivo NR activity (pl-1h-1) was observed maximum in rainy followed by summer and winter in leaf and nodule. Whereas, in the stem and root, maximum activity was observed in summer followed by rainy and lowest in winter. Total NR pl-1 activity was recorded higher in rainy as compared to summer and winter.

Keywords: shisham, seasonal effect, nitrogen, In-vivo nitrate reductase (NR) activity



Terms and Condition Privacy Disclaimer Contact Us

Copyright © GSJ – Global Scholars Journals. All Right Reserved